Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NR2E1

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPSC derived retinal cells
NA
NA

Attributes by original data submitter

Sample

source_name
FLAG-NR2E1
cell type
iRPE

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The cells were collected by scraping and were lysed with lysate buffer, the nuclear pellet was digested by micrococcal nuclease. Immunoprecipitation was performed on the lysate with 2μg of anti-FLAG antibody The DNA fragments were then sequenced using the Illumina HiSeq X-ten platform.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
26430214
Reads aligned (%)
95.4
Duplicates removed (%)
0.2
Number of peaks
186 (qval < 1E-05)

hg19

Number of total reads
26430214
Reads aligned (%)
94.3
Duplicates removed (%)
0.1
Number of peaks
62 (qval < 1E-05)

Base call quality data from DBCLS SRA